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The author reports on the first study to use an audit method to ascertain whether discrimination occurs against queer women (relative to straight women) when they apply to jobs in the United States. A field experiment was conducted in which a pair of fictitious women’s résumés were sent to apply to more than 800 administrative jobs from online job databases advertised by employers across four states. One woman’s résumé was randomly assigned leadership experience at a lesbian, gay, bisexual, and transgender (LGBT) student organization to signal queer identity, while the other résumé, a control, was assigned experience at another progressive student organization. Results reveal that the women with the LGBT indicator on their résumés were discriminated against compared with the other women, receiving about 30 percent fewer callbacks.